FastQCFastQC Report
Sun 14 Apr 2019
SRR6576427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238620
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3860.16176347330483615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.116922303243651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG14550.039.7364771
ACATGGG14300.039.4285133
TACATGG14550.039.4134142
GTATCAA6900.036.7867931
ATGGGAG3050.035.4317975
ATGGGTA1900.034.6210025
ATGGGGG1900.034.6210025
CGAGCCA550.002716781334.171384
CATGGGG5050.033.4947174
TGTACGT300.004164787531.32376542-43
CATGGGA8100.030.1636264
CATGGGC2850.029.6751424
ATCAACG8050.029.183633
CATGGGT2900.029.1635044
TATAAGG650.006153708328.9142423
ATACACC650.006153708328.9142423
AACGCAG8200.028.6497846
CAACGCA8350.028.1351175
TCAACGC8500.027.6386154
TATATTG856.812312E-427.6386135
Produced by FastQC (version 0.11.2)