FastQCFastQC Report
Sun 14 Apr 2019
SRR6576425_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576425_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533833
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.13880745476581627No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6920.1296285542482387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.039.1023671
TAACGCA500.001701518937.5986144
ATAACGC1151.1357406E-732.6944473
ATCAACG15600.030.7296353
TCAACGC16000.029.9613954
CAACGCA16150.029.1010935
GTATAAC1154.0862396E-628.7017351
GTACATG23050.028.6394751
AACGCAG16800.027.6954066
ACATGGG23100.027.6699753
TACATGG24050.027.3637872
TATAACG700.0088253826.8611852
TATCAAC19150.025.2831782
ACGCAGA19450.023.9219977
CATGGGT5500.023.0718774
CGCAGAG20300.022.9203368
GTCTATA1452.4672807E-522.7634471
CTATACT1452.5307088E-522.6888184
TAAGACT1754.4322896E-621.4849224
TAGCACC1354.074123E-420.888124
Produced by FastQC (version 0.11.2)