FastQCFastQC Report
Sun 14 Apr 2019
SRR6576420_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576420_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343315
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7670.2234099879119759No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4470.1302011272446587No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4320.1258319619008782No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4300.12524940652170746No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3890.11330702124870745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.10748146745700013No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3560.10369485749239037No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3450.10049080290695134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA3100.034.8659445
GTACATG24250.033.598471
TACATGG24300.033.456082
ACATGGG24350.032.8084143
CATGGGT4650.032.3394284
GGGTACC3350.032.2640087
TGGGTAC3250.031.81086
ATGGGAG4450.030.6247985
GGTACCT3550.030.4640648
GTATCAA12800.030.170951
ATGGGAT3100.028.8023015
CATGGGA13600.027.9886134
AACGCAG14600.027.3590726
CTAGCTA700.00883375626.8532714
ATCAACG15250.025.268493
GTACCTG4300.025.1505669
TCAACGC15850.024.6084424
CAACGCA16100.024.5182085
CATGGGG8850.024.425864
ACGCAGA16400.024.3562457
Produced by FastQC (version 0.11.2)