FastQCFastQC Report
Sun 14 Apr 2019
SRR6576419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377068
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8890.2357664930463471No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4760.12623717737914647No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4240.1124465613629372No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4050.10740768243393764No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3970.10528604920067468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12950.037.7491651
ATGGGCG1152.764864E-936.7816245
TACATGG26100.033.31772
ACATGGG26500.032.9877933
GTACATG26650.032.9828531
CATGGGT5350.031.6253194
AACGCAG15800.030.0434996
ATGGGTA3200.029.3742125
ATCAACG16050.029.2827033
TCAACGC16100.029.1917654
CAACGCA16200.029.0115685
GTACCGG650.006149800528.9223026
GGGTACC3100.028.805687
GGGGTAT700.00882978526.8564228
AGCGATC700.00882978526.8564228
TATCAAC18100.026.7486862
GGTACCT3350.026.6560028
CATGGGG8650.026.6235644
ACGCAGA18200.025.8234847
CGCAGAG18250.025.7527358
Produced by FastQC (version 0.11.2)