FastQCFastQC Report
Sun 14 Apr 2019
SRR6576414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414290
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.1272055806319245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA353.635816E-667.138634
CGCTAAA353.635816E-667.138635
GTATCAA8800.052.909991
CAACGCA9950.044.3992165
TCAACGC9950.044.3992164
ATCAACG10000.044.177223
AACGCAG10800.040.9048356
TATCAAC11800.038.23952
ATAGCAC959.4359166E-734.6294023
CGCAGAG13150.033.5948458
ACGCAGA13200.033.4675947
TCTTACG600.00415676331.3351442
ATAACTA753.2731605E-431.3313625
TACATGG17450.030.437292
GGTATCA3750.030.0999051
GTACATG18100.029.62181
ACATGGG17850.029.488343
AAGACCG650.00615157728.9212575
GCAGAGT15750.028.3474229
CCCGCTA856.8111846E-427.6453193
Produced by FastQC (version 0.11.2)