FastQCFastQC Report
Sun 14 Apr 2019
SRR6576413_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576413_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293712
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9390.319700931524759No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5700.19406765811407092No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4850.16512774418477966No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4770.16240398757966987No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4570.15559459606689546No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3620.12324998638121698No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3620.12324998638121698No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3610.12290951680557825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.12120716892738465No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3500.11916435147355231No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3390.1154191861415264No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3390.1154191861415264No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3170.10792885547747454No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3160.10758838590183581No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2990.10180040311597756No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2950.10043852481342266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC301.3704888E-462.6572463
ATCTATA352.9336152E-453.7153552
GTATCAA7250.044.181441
GTCCTAA500.00168139237.6841621
TAACCCG500.001701221337.5943455
GTACATG15200.036.8784181
TATAACC651.4121778E-436.148413
TACATGG15700.035.924282
ATCAACG9250.035.562223
CAACGCA9150.035.4372945
TCAACGC9400.034.994744
ACATGGG16250.034.7024733
AACGCAG9300.034.3604286
TTCATAC550.00271573234.176683
GGTACCT3100.031.8556148
CATGGGG8300.031.7060744
TAGACAT600.004159044531.3286235
GGGTACC3200.030.8391137
TAAGACA1401.8835635E-830.2097434
ATGGGTA3450.029.9665095
Produced by FastQC (version 0.11.2)