FastQCFastQC Report
Sun 14 Apr 2019
SRR6576413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293712
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8900.30301792231846164No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5550.1889606144794901No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4880.16614915291169582No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4580.15593506564253418No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4490.1528708394617857No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4150.14129487389006917No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3950.13448548237729477No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3900.13278313449910117No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3710.12631421256196546No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3520.11984529062482975No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3200.1089502642043907No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2970.10111946396470012No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2970.10111946396470012No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2950.10043852481342266No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2940.10009805523778395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6100.043.1570781
ATAGACG450.001014167141.7656753
AGTAATA704.616426E-640.294622
TACATGG16600.037.9481472
GTACATG16650.037.8341871
TATATAC500.001702387137.5891043
ACATGGG17000.037.5891043
GGGTACC3400.035.9307637
TCTACAC1055.102993E-835.799153
ATGGGTA3200.035.2397845
TGGGTAC3550.034.4125636
TAGACGT550.0027175934.1719134
GGTATAT702.178227E-433.578851
TGGGAGT1309.164069E-932.529036
CATGGGT5100.031.3242554
TTATATA600.00416188531.3242554
GGTACCT3800.030.912098
CATGGGA5750.029.417564
TATACTG1602.1263986E-929.3664865
AACGCAG8650.029.3325395
Produced by FastQC (version 0.11.2)