FastQCFastQC Report
Sun 14 Apr 2019
SRR6576411_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576411_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253886
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4100.16148980250978787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.11816327012911305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2920.11501224959233672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG6450.037.881474
GTACATG17800.036.5076831
TACATGG18150.035.4672162
ACATGGG18450.034.3811653
AGTATTG550.002716555534.1727945
GTATCAA9250.034.1081541
ATGGGGG2250.033.41345
GTACTTT8550.031.9438321
CATGGGC3100.031.8303034
ATTCGCA300.00416017831.33123482-83
AACGCAG10150.031.016446
ACTTTTT9050.029.0751953
TCAACGC10700.028.9830024
ATCAACG10550.028.9497013
CAACGCA10900.028.8822785
TACTTTT9700.028.5800842
ACGCAGA11400.028.0332097
GTATTAG1354.5345405E-727.9049591
CGCAGAG11600.027.5661688
ATGGGGA3550.026.4718825
Produced by FastQC (version 0.11.2)