FastQCFastQC Report
Sun 14 Apr 2019
SRR6576410_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576410_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283973
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4480.15776147732354837No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.12606832339694266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3450.12149042338532184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG20450.039.8499031
TACATGG20250.039.701162
ACATGGG19850.039.5537873
GGGTACC2650.033.696667
TAGGACA851.7464528E-533.186734
CATGGGG6600.032.7677044
CATGGGT4750.032.6627274
GGTACCT2750.032.471338
ATGGGAG4350.032.4238135
ATGGGTA3700.031.7665775
CATGGGA11700.031.7448564
GGTATGC600.004110255731.4038721
GTATCAA9800.030.7629761
ATGGGAT2850.029.693395
AACGCAG11350.026.9246226
ATAGACT1057.386683E-526.8654464
ATCAACG11250.026.7460463
CAACGCA11650.025.8277265
TCAACGC11650.025.4241684
TAGCGCG1151.3707642E-424.529324
Produced by FastQC (version 0.11.2)