FastQCFastQC Report
Sun 14 Apr 2019
SRR6576410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283973
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4970.17501663890581148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.10388311564831867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG18450.037.6997762
ACCGTCG757.4267064E-637.597898
GTACATG19100.037.401041
CATGGGG7000.035.5837144
CCGTCGT801.1560285E-535.248029
ACATGGG19800.035.1293373
ATGGGAG3650.029.6147735
CATGGGA10700.029.4282534
TACACCC804.7734956E-429.373354
TACCGTC804.7734956E-429.373357
CATGGGT3600.028.720614
TAGGACA1154.18361E-628.6070884
ATGGGGA3650.028.3271755
GTATCAA8800.028.3052271
GTCATAG856.8056013E-427.6455061
CTAGACA856.8056013E-427.6455064
ATCTTCG700.00882744326.8556337
GCGTGTA350.00883474726.85563330-31
GTATAGG1101.0160814E-425.6349221
GACCGCG1301.0845444E-525.3062697
Produced by FastQC (version 0.11.2)