FastQCFastQC Report
Sun 14 Apr 2019
SRR6576407_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576407_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191384
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.24296701918655686No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3250.16981565857124942No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2380.12435731304602266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.11442962839108807No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2170.11338460895372654No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2100.10972704092296116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGC558.734005E-751.3051038
GTATCAA7650.043.0336951
CATGGGT4000.042.2935334
GGGTTAG704.61984E-640.2795527
CATGGGG4800.040.1396944
ATGGGTG1650.039.8726925
GTACATG15900.039.043511
TACATGG15950.038.900772
ACATGGG16050.038.6482933
TGGGTTA801.154826E-535.244616
GTAGGAC550.00271341334.176593
CTATAGT702.1701629E-433.592631
ATATAGC702.1801873E-433.5662963
TATAGTA851.7458862E-533.1800652
AACGCAG10300.032.8493466
GGTACCT1755.456968E-1232.2489248
ATCAACG10300.031.9368653
TCAACGC10450.031.4784374
ATGGGTA2100.031.3285435
ATGGGGG2700.031.3285435
Produced by FastQC (version 0.11.2)