FastQCFastQC Report
Sun 14 Apr 2019
SRR6576404_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576404_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240627
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3920.16290773687075846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.12010289784604387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6500.043.5188371
CATGGGG6300.035.808344
TACATGG14900.035.3276862
GTACATG15150.035.164561
ACATGGG15050.034.66333
AACGCAG9350.032.6727146
ATGGGTA2050.032.0964975
GGTATCA3300.031.430271
TGGGTAC1658.54925E-1131.3322946
TTATGCA600.0041551831.3322944
TCAACGC9450.031.3322944
CAACGCA9600.030.8427285
ATCAACG9600.030.353163
ATGGGGA3450.029.970025
CATGGGT3800.028.4464244
TATACAG1005.3075026E-528.1990625
TAAGGTA856.801642E-427.6461414
TAGGACC1057.3993E-526.8562534
ACGCAGA11050.026.7954927
CGCAGAG11250.026.3191288
Produced by FastQC (version 0.11.2)