FastQCFastQC Report
Sun 14 Apr 2019
SRR6576404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240627
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3770.15667402244968354No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2640.10971337381091897No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.10431082131265404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6800.049.7636031
ATCAACG8250.041.586963
TACATGG13850.040.7211232
TCAACGC8350.040.526054
ACATGGG13950.040.429213
AACGCAG8500.039.8108866
GTACATG14200.039.386451
CAACGCA8600.039.347975
GTCCTAT906.164264E-736.554751
CATGGGG5500.035.8901184
ACGCAGA9500.035.6202667
TATCAAC9750.034.7069242
CGCAGAG9700.034.4013028
CATGGGT3400.033.1757354
ATAGTGT753.2683322E-431.3326426
GCAGAGT10800.030.8974679
CTTACTC804.770403E-429.374353
CCTTACT1154.1790117E-628.608062
GTGTATA1005.307101E-528.1993771
CCTATTC1503.6730853E-828.1993773
Produced by FastQC (version 0.11.2)