FastQCFastQC Report
Sun 14 Apr 2019
SRR6576403_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576403_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560338
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10790.1925623462981272No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.13259853873911817No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6160.10993364719151653No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6080.10850593748773063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10700.051.9846461
ATACGGG503.0106934E-546.991393
TCAACGC14350.038.3135384
ATCAACG14800.037.4661063
CAACGCA14750.037.2745255
AACGCAG15450.035.8898666
TACATGG26200.034.625062
GTACATG26350.034.5265771
ACATGGG27300.032.876763
GGTATCA5000.030.1687151
TATCAAC18500.029.7268372
ACGCAGA18950.029.2611277
GTATACG650.00606362829.008381
TATAGAC1005.325376E-528.1948343
CATGGGG18750.027.5682814
CAAACGC700.00884012326.8522224
CGCAGAG20800.026.6585758
ATAACGC1458.6510227E-725.9262853
GTATTAG1151.348741E-424.594061
GAATAAC1951.6983904E-824.173651
Produced by FastQC (version 0.11.2)