FastQCFastQC Report
Sun 14 Apr 2019
SRR6576400_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576400_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407746
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12170.2984701260098198No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8120.19914358448642047No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7680.1883525528147425No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7360.1805045297807949No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7280.17854252402230802No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6290.1542627027610326No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5910.14494317540821983No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5680.13930240885256998No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5650.1385666566931374No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5540.1358688987752179No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5450.13366164229692015No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC5420.13292589013748757No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5170.12679462214221598No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5090.12483261638372908No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4660.11428683543186201No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4610.11306058183280768No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4590.11257008039318596No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4560.11183432823375337No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4500.11036282391488819No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4460.10938182103564474No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG4210.10325055304037317No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4210.10325055304037317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.10202429944131887No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4130.10128854728188627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG24050.037.1855161
GTATCAA10900.037.1370351
CATGGGG10850.036.3824424
ACATGGG24750.035.3166963
TACATGG25300.035.2919272
GTATACT851.734504E-533.22521
GTACTAG753.2438157E-431.3793561
CTATTAA1501.0495569E-931.3793561
AACGCAG13100.029.7748186
GTATATA804.7346606E-429.4181461
ATATAAC804.779111E-429.3712443
TCAACGC13500.028.8926034
CAACGCA13550.028.7859885
ATCAACG13800.028.6050383
GTACTTT10100.027.9618031
TTTAGTA856.813583E-427.6435242
CTTAGGG1755.5388227E-926.8537083
CTCTACT1057.4164855E-526.8537084
GTCGAGT350.00884005526.85370662-63
TCTTAAG700.008834965526.8537062
Produced by FastQC (version 0.11.2)