FastQCFastQC Report
Sun 14 Apr 2019
SRR6576392_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576392_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385417
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9440.2449295178987953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.20004307023302031No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6330.16423769579442526No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5820.1510052748062488No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5430.14088636463881976No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5270.13673501687782322No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5240.1359566391726364No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5130.1331025875869513No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4910.12739448441558104No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4870.12635664747533193No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4720.12246475894939765No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4550.11805395195333886No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4390.11390260419234233No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC4210.10923233796122121No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4130.10715666408072294No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG4030.10456207173010013No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3950.10248639784960185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT450.0010133541.7758188
CTACGCT450.0010133541.7758184
GTATCAA16500.039.7571331
TATACCC651.4121366E-436.1521535
CCTATAC550.002715403434.1802143
ATCAACG19200.033.7796673
TCAACGC19150.033.6224444
CAACGCA19350.033.274925
AACGCAG19800.032.99346
GTCGCTG600.00415853731.3318638
CGATCTA300.004161094331.33186372-73
GTACATG26450.031.2245771
ACATGGG26750.030.5705263
TACATGG26650.030.5168082
ACGCAGA21950.029.9758137
TCTACAC1154.188414E-628.6073533
CGCAGAG22750.028.508558
ATTAGGC1005.3161843E-528.1986773
ATATCTA856.8096805E-427.6457616
GGACCGA856.8096805E-427.6457616
Produced by FastQC (version 0.11.2)