FastQCFastQC Report
Sun 14 Apr 2019
SRR6576391_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576391_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224310
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4060.18099950960723998No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.15291337880611655No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2710.12081494360483261No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2360.10521153760420845No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2250.1003076100040123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7550.041.765971
GAGTAAT651.3985086E-436.2035681
TATAGCC651.4114208E-436.147072
AACGCAG9200.034.221856
TCAACGC9650.033.1129654
CAACGCA9600.032.795945
ATCAACG9600.032.795943
ATGGGAG2800.031.8868835
TATCAAC10500.029.9848562
TAGACAG953.7437585E-529.6786485
TACATGG14100.029.661112
ACGCAGA10700.029.4243937
ATATAGC650.00610262728.9628521
AGTAATG650.00614938328.917662
CGCAGAG11100.028.3830348
GTACATG14600.028.3677231
ACATGGG14800.027.6232013
CATGGGA7000.027.5234134
CACGAAC350.00883365126.85510442-43
GCAGAGT11950.026.3641599
Produced by FastQC (version 0.11.2)