FastQCFastQC Report
Sun 14 Apr 2019
SRR6576391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224310
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5320.23717177120948685No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3070.13686416120547457No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2700.12036913200481478No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.11903169720476126No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2570.11457358120458294No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2540.11323614640452945No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2290.10209085640408365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC200.002160967870.497831
CCGCTAA200.0021628870.482124
CGGATAG200.0021628870.482126
CAAACGC408.014413E-658.748192
ACCCGTA250.005235818756.3856937
GCAAACG558.780862E-751.271151
GTATCAA5900.047.795141
TAGGGGT405.673989E-446.9880754
CTAGGGG405.673989E-446.9880753
GTACATG14300.043.3832781
CATGGGT5350.043.035814
TACATGG14300.042.7259562
ACATGGG15350.039.4883463
AGTCGTA250.001691881737.64080444-45
ACTATCC500.001701227737.590468
TTAGTCG250.001703042737.5904642-43
AACGCAG7650.037.4676176
CCTTATA651.410121E-436.1527332
TCAACGC7950.036.0537454
CAACGCA8100.035.386085
Produced by FastQC (version 0.11.2)