FastQCFastQC Report
Sun 14 Apr 2019
SRR6576390_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576390_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246958
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4980.2016537224953231No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3440.13929494084014288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.134030887843277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGC450.00101105641.789126
GTATCAA7850.041.432421
TACATGG15050.039.359522
ACATGGG15800.037.788743
GTACATG16100.037.7680631
TACCCCT550.002709288434.1910975
ATGGGAG3500.033.5805445
AACGCAG9700.032.9573976
CAACGCA9750.032.7883875
CATGGGA8050.032.120524
ATCAACG10100.031.6521573
TCAACGC10100.031.6521574
TTACCCC600.00414919731.3418394
ACGGTAT753.270717E-431.3291519
ATAGTCC902.5881105E-531.329158
GTTTAAT953.6669437E-529.770661
GACGGTA804.7738763E-429.3710778
TACGACG650.006136793628.930935
CATGGGG6050.028.7516064
ACGCAGA11250.028.4050967
Produced by FastQC (version 0.11.2)