FastQCFastQC Report
Sun 14 Apr 2019
SRR6576387_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576387_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243438
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6060.24893402016119096No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.15363254709618054No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3130.12857483219546662No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3060.1256993567150568No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2850.11707293027382742No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2810.11542980142787898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2570.10557102835218825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACGG407.982118E-658.79073394
ATAACGC405.671457E-446.9939423
GTATCAA7550.041.737531
GGTTAGC757.3946794E-637.618348
CGCGATA250.001702141637.5951582-83
GCGATAT250.001702141637.5951584-85
GTACATG13050.037.4819071
CATGGGT3400.037.3187144
TACATGG13350.036.969122
AACGCAG8350.036.5821086
ACATGGG13150.036.0942083
CAACGCA9000.033.9400675
GTATAAA1256.182745E-933.863461
TCAACGC9050.033.7525564
ATTGACG352.1742893E-433.59470492-93
GCCTAGT851.7408498E-533.199471
CTAGTAC1151.1323391E-732.6914373
ATGGGTA2450.032.608045
ATCAACG9600.031.8188133
TCCGACC753.2586843E-431.3486168
Produced by FastQC (version 0.11.2)