FastQCFastQC Report
Sun 14 Apr 2019
SRR6576386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268569
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5800.215959399632869No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3480.12957563977972142No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2970.11058610636372776No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2790.10388391809925941No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2770.10313923051431848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7150.045.3551141
ATCAACG8550.035.1801033
TCAACGC8700.034.573554
CAACGCA8750.034.3759845
AACGCAG8800.034.1806686
GTACATG14850.032.2817421
CAAACGC600.004156043331.3322772
TACATGG15250.030.8186322
ACGCAGA9800.030.6928467
ACATGGG15050.030.603623
TATCAAC10700.030.3073922
ATGGGTA2650.030.1499275
GATATAC650.00614689828.9221063
GCATATA650.00614689828.9221061
CGCAGAG10600.028.3764028
ATAACGC1005.309785E-528.199053
GGTACCT2950.027.0838348
GGGTACC3200.026.436617
CATGGGA5350.026.3542544
ATAATAC909.5005013E-426.110233
Produced by FastQC (version 0.11.2)