FastQCFastQC Report
Sun 14 Apr 2019
SRR6576384_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576384_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526354
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16790.3189868415553031No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9590.18219677251431546No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9200.17478731044126197No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8600.16338813802117969No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7800.14818924146106993No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7360.13982984835300957No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7350.1396398621460082No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG7030.1335603035219643No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6790.12900063455393138No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6150.11684151730584359No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5860.11133191730280381No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5780.10981202764679283No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5760.1094320552327901No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5740.10905208281878735No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5730.10886209661178597No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5520.10487238626475717No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5410.1027825379877421No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC5380.10221257936673797No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5370.10202259315973661No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG5310.10088267591772837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11600.043.50391
GTACATG27200.037.106271
ACATGGG27400.035.851743
TACATGG28400.035.416852
ATGGGTA6850.034.9940455
CATGGGT9250.034.552684
TCAACGC15050.033.4165654
CAACGCA15250.032.9783135
ATCAACG15850.031.7299273
AACGCAG15950.031.530996
GGTACCT6400.030.8420058
CATGGGG11500.028.6097934
TGGGTAC7400.027.9470186
GGGTACC7250.027.8742857
ATGGGAG5350.027.234695
ACGCAGA18900.027.1042847
GTATAAC909.320372E-426.2017271
CGCAGAG19600.025.6567128
CTATTCA1701.2418604E-724.8809459
GTATTAC1151.343842E-424.6068381
Produced by FastQC (version 0.11.2)