FastQCFastQC Report
Sun 14 Apr 2019
SRR6576379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229299
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7610.3318810810339339No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4840.21107811198478843No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3850.1679030436242635No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3820.16659470821939912No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3510.15307524236913375No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3350.14609745354319034No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3070.13388632309778936No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2850.12429186346211715No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2850.12429186346211715No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2850.12429186346211715No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2720.11862241004103813No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2540.11077239761185177No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC2530.1103362858102303No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2520.10990017400860884No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2460.10728350319888005No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2450.10684739139725861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGC450.001013551441.7669221
TGAGTAC450.001013551441.7669223
CATGGGG6250.039.0938384
GTACATG14050.038.7941861
TACATGG14200.038.715292
GTATCAA6200.037.893381
ATAGTCC500.001701357537.590233
ACATGGG14650.036.884613
CCTAATA550.002715953634.1729352
CATGGGT3800.032.1495364
AACGCAG7150.031.5442476
ATCAACG7300.031.5397473
TCAACGC7300.031.5397474
TTGGACA753.2724143E-431.325194
TATATGA600.00415938731.325192
TACTATA600.00415938731.325192
CAACGCA7400.030.4785655
GCTATAT804.776354E-429.3673653
CTATATA1452.6471753E-829.1648334
ACTATAT650.006151842428.915563
Produced by FastQC (version 0.11.2)