FastQCFastQC Report
Sun 14 Apr 2019
SRR6576377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479171
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16570.3458055683670339No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9850.20556335838354156No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA9270.1934591200218711No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8760.18281573801419534No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8760.18281573801419534No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7010.14629432916432755No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6670.13919874115921038No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6480.13523355962693903No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG6350.13252054068380598No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC6290.13126837809466768No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6150.12834666538667824No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT6140.12813797162182186No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6060.12646842150297077No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5620.11728589584928972No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5480.11436418314130029No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5270.10998161407931616No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5080.10601643254704478No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5030.10497296372276287No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC5020.10476426995790647No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG4940.10309471983905537No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC4880.10184255724991703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11250.048.8878171
TTAGGTA1253.6379788E-1241.3493464
TATTAGT802.4595283E-741.1144074
TAGGTAT1251.5097612E-1037.5903175
TATATAC500.001703190237.5903173
ACCGTAC500.001703190237.5903177
GGTATAG1302.3101165E-1036.1445357
TCAACGC14800.035.8758934
AACGCAG14950.035.2016336
CAACGCA15150.034.7369275
ATCAACG15350.034.590443
TACATGG27150.033.9354442
GTACATG27650.033.831811
TTATTAG851.755748E-533.1679273
AGGTATA1457.4396667E-1032.4054456
ACATGGG28300.032.376823
TATAAGA1252.3550274E-730.084812
GGTACCT5000.030.0722528
CTTAGGT1952.0008883E-1128.9156283
GTAACCC1303.3322613E-728.9156283
Produced by FastQC (version 0.11.2)