FastQCFastQC Report
Sun 14 Apr 2019
SRR6576376_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576376_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535159
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15630.292062732757928No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10570.19751139381006394No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9050.16910862005497432No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8780.16406339050637286No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8430.15752327812855618No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7120.1330445718001566No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6800.12706504048329562No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6720.12557015765408036No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6490.12127236952008656No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6480.12108550916643465No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6350.11865632456895987No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5940.11099505006923177No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5830.1089395861790608No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5650.10557609981332651No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5590.10445493769141508No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5560.10389435663045936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA250.005232167456.406395
GTATCAA12050.040.6982231
GTACATG28650.037.3620221
TACATGG28900.036.2705462
ACATGGG29450.034.6355063
TAGGTAT1554.1836756E-1133.358625
AGGTATA1606.002665E-1132.3161626
CATGGGG15350.031.5410334
TCAACGC15350.031.234814
AACGCAG15350.030.9285876
TGGGTAC6550.030.858466
ATCAACG15650.030.636063
ATGGGTA6600.030.6246815
TTAGGTA1701.2187229E-1030.415214
CTTAGGT1701.2187229E-1030.415213
CAACGCA15800.030.3452115
GGGTACC6700.030.161967
GGTACCT6550.030.1351878
TATTATC2500.030.0834082
CCTATAT804.6861873E-429.471971
Produced by FastQC (version 0.11.2)