FastQCFastQC Report
Sun 14 Apr 2019
SRR6576375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163989
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3050.18598808456664775No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2410.14696107665758068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.12561818170730962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGC701.4115358E-953.7008675
GTATATA952.1025699E-839.569061
ATGGGCG608.803958E-539.1568875
ATGGGAG3400.038.6962135
CATGGGG4000.037.5906074
ACATGGG14500.035.6462673
TACATGG15000.035.397822
GTACATG15500.034.5591051
GTGTTAA702.1800706E-433.563041
GCCCCTA300.004106146331.41172450-51
CATGGGC2550.031.3255084
ATATATG1201.641256E-731.3255083
TGGGAGA1751.6916601E-1029.5354796
TCTTGTA804.7702028E-429.3676622
GGAGTCC650.00614649928.915858
ATGGGCA1652.932211E-928.4777325
GTATCAA8300.028.3061811
CATGGGA8800.028.2997474
TGGGAGT2400.027.409826
GTGTAAG700.0088250926.8504331
Produced by FastQC (version 0.11.2)