FastQCFastQC Report
Sun 14 Apr 2019
SRR6576373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337065
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11910.353344310444573No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7100.21064186432883866No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5600.16614006200584458No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5250.15575630813047928No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5100.15130612789817988No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5020.14893269844095353No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4650.13795558720128165No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4420.1311319775117559No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4240.12579176123299662No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4230.1254950825508433No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4230.1254950825508433No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4000.11867147286131756No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3820.11333125658255826No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3820.11333125658255826No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3740.1109578271253319No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3680.10917775503241214No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT3560.10561761084657262No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3510.10413421743580614No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA3510.10413421743580614No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG3440.10205746666073309No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3400.1008707519321199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT4900.036.4389724
GTACATG20050.036.1059071
ACATGGG19800.036.07093
TACATGG20500.035.3133352
CCTATAG801.1584019E-535.240323
GGTACCT3250.034.6981628
GTATCAA9000.033.427921
ATGGGTA3550.031.7659235
ATATGAG1201.6503873E-731.324735
TGGGTAC3550.030.4423436
CATGGGG8800.030.4348224
GGGTACC3650.029.6083077
CGGACTT650.006156337428.9151335
TAGTACT856.816962E-427.6394674
AACGCAG11000.027.3379446
ATGGGGA4850.027.1265685
GTACCTG4000.027.017589
CTATAGA700.00883915426.849774
TCAACGC10850.026.8497684
CAACGCA10950.026.6045655
Produced by FastQC (version 0.11.2)