FastQCFastQC Report
Sun 14 Apr 2019
SRR6576372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373921
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12930.3457949673861591No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6910.1847983932434926No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6230.16661273370578278No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5530.14789220182872853No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5460.1460201486410231No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4790.12810192527298547No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4570.122218329540197No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4530.12114858486150819No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4510.12061371252216377No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4410.11793935082544174No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4300.11499755295904748No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4190.11205575509265325No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4090.10938139339593124No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4020.10750934020822579No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3990.10670703169920918No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT3890.10403267000248714No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3880.10376523383281495No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG3860.10323036149347053No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3850.10296292532379833No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3760.10055599979674852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTTA250.001701849537.5990926-27
GTACATG22100.037.428961
TACATGG22250.037.176632
ACATGGG21950.037.0423853
GTATCAA10200.036.4010771
CATGGGG9700.032.463134
CTATAAG1201.647586E-731.3325771
ATACTAC600.00415791631.3325776
AACGCAG11850.030.9359596
ATGGGTA3550.030.4499685
CAACGCA12200.029.6632165
ATCAACG12250.029.5421433
TAACACA804.775462E-429.374294
TCAACGC12350.029.3029374
CATGGGA10400.028.9223774
CTAGTAC650.006149658528.9223773
TGGGTAC4100.028.6578436
CATGGGT5750.028.6080044
AACGAAC1154.1872336E-628.60800494
GGTACCT4250.027.6463898
Produced by FastQC (version 0.11.2)