FastQCFastQC Report
Sun 14 Apr 2019
SRR6576369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271731
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.1847415274665018No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2820.10377910507082372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG156.8727444E-494.08161
GTATCAA9700.041.7062761
ACGCAAA853.9424413E-738.703845
AACGCAA959.419309E-734.629754
ATCAACG11700.033.340123
CTAGTAC1001.4064954E-632.8982623
TCAACGC11950.032.6426244
CAACGCA12400.031.8370275
TATCAAC12300.031.7196082
CGCAAAA1052.0585503E-631.3316786
TAGACCC600.004156518331.3316764
TCCGAAC300.004160145831.33167672-73
AACGCAG12750.030.9630686
GTACATG17850.028.9887261
TTAGCAC650.006147628.9215473
TACATGG17950.028.5441342
ACGCAGA14350.027.5107427
GCAAACG1057.357491E-526.8804571
GTATACG700.00878709326.8804571
ACATGGG18350.026.6361923
Produced by FastQC (version 0.11.2)