FastQCFastQC Report
Sun 14 Apr 2019
SRR6576368_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576368_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328295
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5590.1702736867756134No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3850.11727257497068186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.10204237042903486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG22400.038.8834571
TACATGG22600.038.6768382
ACATGGG22850.038.2478523
GTATCAA10600.031.9792141
GTATAGC1401.8511855E-830.2660431
CATGGGG8550.030.2257374
TATACTA804.7811013E-429.3670485
CATGGGA11600.029.1645184
TATGCTA650.00615598728.915255
CATGGGC4550.028.9152494
AACGCAG11100.028.7850026
GTATAAG1005.2504867E-528.2483061
CATGGGT5750.027.7837834
ACTAGAT700.00881241326.8662458
CTTAGAC1057.41936E-526.8498763
ATCAACG12300.026.3587193
GGTACCT2500.026.3289188
TCAACGC12500.025.936984
ATGGGTA3650.025.7464565
CAACGCA12600.025.3582155
Produced by FastQC (version 0.11.2)