FastQCFastQC Report
Sun 14 Apr 2019
SRR6576368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328295
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6570.20012488767724151No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3590.10935286860902542No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3410.10386999497403249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG23950.042.1917272
GTACATG24350.041.498641
ACATGGG24300.040.411233
CATGGGT5550.038.096194
ATGGGTA3450.036.7711075
GTATCAA10550.036.530511
ATGGGAG5100.035.0086565
GTATTAC702.1817186E-433.5711561
CATGGGG9100.032.5282864
GGTACCT2900.032.4036568
CATGGGA12650.032.3140034
GGGTACC3200.030.8341037
AACGCAG12750.030.586516
ATGGGGG3150.029.8319385
TCAACGC13150.029.656124
ATCAACG13250.029.43233
CAACGCA13300.029.3216535
ATAATAC1154.194597E-628.5997493
TGGGTAC3500.028.1911816
ACGCAGA14650.026.6196587
Produced by FastQC (version 0.11.2)