FastQCFastQC Report
Sun 14 Apr 2019
SRR6576367_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576367_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360164
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5770.1602047955931187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10800.037.043431
TACATGG23750.036.2159652
GTACATG24100.035.9349331
ACATGGG24350.034.7445073
CATGGGG9700.031.011364
AACGCAG13150.030.7386866
TCAACGC13550.029.8312724
CAACGCA13600.029.7215965
ATCAACG13650.029.6127283
AAACTCG650.006147612428.9240635
CATGGGA11700.027.7188914
ATGGGTA2950.027.0856655
CATGGGC4350.027.0124114
TGTGCGA350.00883838926.85432810-11
ACGCAGA14900.026.8129927
CGCAGAG15100.026.4578538
TATCAAC15300.026.4191992
GTATGCC909.503061E-426.1119983
CTAAGAC1301.0848396E-525.3085543
ATGGGGA5150.024.6416155
Produced by FastQC (version 0.11.2)