FastQCFastQC Report
Sun 14 Apr 2019
SRR6576367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360164
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6380.17714152441665462No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3620.10050976777245921No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACG250.005234199656.3970875
TACATGG24500.037.7898862
GTATCAA9950.037.786991
GTACATG25000.037.410071
ACATGGG25300.035.8519023
CATGGGG7650.031.3317134
CATGGGA13400.030.8640754
AACGCAG12350.030.0632236
CATGGGT5800.029.981214
TCAACGC12350.029.3021284
AAGACCG650.00615018828.9215815
ATCAACG12600.028.720743
CAACGCA12900.028.4171355
GGCTAAT1005.3154017E-528.1985441
ATGGGAG5800.027.55035
ATGGGAT3750.026.318645
ACGCAGA14500.025.6055747
TATCAAC14800.025.4040932
TACGCTA2101.4224497E-924.6177739
ATATACG2101.4224497E-924.6177736
Produced by FastQC (version 0.11.2)