FastQCFastQC Report
Sun 14 Apr 2019
SRR6576365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337512
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7960.23584346630638317No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4520.13392116428452913No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4190.12414373414871174No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4170.12355116262532886No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4010.11881059043826589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.10192230202185405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGCG500.001700324437.6000027
GTACATG22450.036.008911
GTATCAA11800.035.050851
ACATGGG23400.034.7478643
TACATGG23550.034.526542
CATGGGG8000.034.0754
GGTCTAA851.7511278E-533.176471
ATGGGTA3650.029.616445
ATCAACG14050.029.1032053
AACGCAG14550.029.0721666
TATACTC650.00614809328.9230774
TCAACGC14200.028.7957744
TATACAG1802.3646862E-1028.7222215
CAACGCA14600.028.6506865
GCCCTAA856.8060454E-427.647061
ATGGGGA4550.026.8571455
CATGGGT5600.026.8571434
CAATCGC350.00883348626.85714378-79
ATTATAC909.5037074E-426.111113
ACGCAGA16400.025.7926837
Produced by FastQC (version 0.11.2)