FastQCFastQC Report
Sun 14 Apr 2019
SRR6576362_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576362_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272901
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5350.1960417880476803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.14510756648015213No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3200.11725863957992091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA250.00523641956.3873564
ATAATAC555.2794487E-542.7176973
GTATCAA8950.041.0193521
TACATGG18550.036.4769972
GTACATG18750.036.1475141
CATGGGT4500.035.503154
ACATGGG19100.035.4266133
AACGCAG11000.033.31986
ATCAACG11050.032.7437863
CATGGGA10100.032.1017154
TCAACGC11300.032.0193674
CAACGCA11500.031.8711155
GGTTAGC600.004141412731.3550348
TAGCGCG1052.061348E-631.326314
TAATGCG300.004163651331.3263118-19
CTATTAA804.7317497E-429.4169241
AGGCGTG804.777711E-429.3684167
AAAGGCG804.777711E-429.3684165
TATCAAC12400.029.1789442
ACGCAGA12600.029.0887157
Produced by FastQC (version 0.11.2)