FastQCFastQC Report
Sun 14 Apr 2019
SRR6576361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296519
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5870.19796370552983114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.12747918345873283No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3470.11702454142904839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7800.048.8142621
TACATGG18850.040.647382
GTACATG19550.039.672861
ACATGGG20100.038.113133
CACGATA500.001700275837.5987294
ATCAACG9750.037.598723
TAACCGG250.001701647837.5987250-51
CAACGCA9800.037.4068875
AACGCAG9950.036.8429686
TCAACGC10000.036.6587524
GTCCTAT906.1631545E-736.5604741
ATGGGTA1950.036.1526155
ATGGGAC2700.034.8136255
CATGGGT3800.034.6303984
CATGGGA12350.033.869294
TGGGTAC2000.032.898886
ATGGGTG1755.456968E-1232.2274745
TATCAAC11750.032.0043072
ACGCAGA11650.031.4667387
CGCGGTA1201.645767E-731.33226694
Produced by FastQC (version 0.11.2)