FastQCFastQC Report
Sun 14 Apr 2019
SRR6576360_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576360_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158296
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.289963107090514No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3470.21920958204881993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.14656087330065193No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2170.1370849547682822No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2150.13582149896396625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2120.1339263152574923No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1800.11371102238843685No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1780.11244756658412088No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1700.10739374336685703No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1650.10423510385606712No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1620.10233992014959316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAC250.00517227756.552091
GTACTGA500.001698886637.5941856
CGCCGGT500.001698886637.5941857
GTATCAA9250.035.663481
TACATGG18750.035.087912
GTACATG18900.034.659351
GGTACCT1107.5930075E-834.1981438
CATGGGT3200.033.7760284
ACATGGG18850.033.6552773
TACAGTC702.1785099E-433.566247
GGGTACC1309.094947E-932.5334327
CATGGGG4800.032.3075034
ATGGGAG3400.031.7892025
GGTCGCC300.00414034931.35821392-93
GCCGGTC600.00414055231.3482998
CTAAGAC650.00614300228.9186063
CATGGGA11150.027.8163264
TTGTACA856.796104E-427.6427844
GCTGTGC1205.78003E-627.4297628
TGGGTAC1555.0329618E-827.2861046
Produced by FastQC (version 0.11.2)