FastQCFastQC Report
Sun 14 Apr 2019
SRR6576358_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576358_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405341
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.17170727856298773No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5530.13642834058237385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8850.048.5245821
CTTAGCG601.6027061E-646.999323
TTAGCGC608.81472E-539.16614
TCAACGC10900.037.513224
CAACGCA11000.036.7449235
ATCAACG11300.036.1853183
TAGCGCC651.4119956E-436.153325
AACGCAG11650.034.694786
GGTATCA4250.034.4220281
TATCAAC12900.032.4419022
CATGGGT4400.032.044994
ACATGGG16500.031.6177253
TACATGG16500.031.3483542
CCTATAC753.2721052E-431.332883
GTACATG16650.031.177561
ACGCAGA13950.028.9709177
ATGGGTA3250.027.4765265
CGCAGAG14900.027.1237778
ATTATAC1258.008061E-626.319623
TGGGTAC3600.026.1107356
Produced by FastQC (version 0.11.2)