FastQCFastQC Report
Sun 14 Apr 2019
SRR6576355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394721
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8730.22116887624423326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.1388322384671705No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5110.12945852893562793No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4880.12363162841602043No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4530.1147646058861829No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4140.10488420935293537No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4130.10463086585208287No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4060.10285746134611536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11050.048.0557061
ATGGGCG708.608913E-846.9865765
TACATGG27250.042.595062
GTACATG27550.042.472371
ACATGGG28000.040.9454423
ATCAACG13450.038.7770233
TCAACGC13800.038.134034
CAACGCA13950.037.7239885
AACGCAG14000.037.5892566
CATGGGA14800.033.970024
ACGCAGA15900.032.801957
CATGGGG8950.032.549364
TGGGTAC3500.032.2193646
ATGGGTA3800.032.148715
TGCCCGC753.2747287E-431.328351
CGCAGAG16700.031.2305978
ATGGGAT5750.031.0519945
GGGTACC3400.030.4030767
CATGGGT6250.030.0714054
GTCTAGA953.750171E-529.6794911
Produced by FastQC (version 0.11.2)