FastQCFastQC Report
Sun 14 Apr 2019
SRR6576353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155903
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4430.28415104263548485No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2620.1680532125744854No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2260.14496193145738054No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.14175480908000487No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2200.14111338460452974No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1880.12058780138932543No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1770.11353213215909892No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1770.11353213215909892No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCTTACATCTCGTATGCCGT1740.11160785873267351No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1680.10775931187982271No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1630.10455218950244703No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1610.10326934055149677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC550.085.485762
CCGAACT255.5342804E-575.227471
CCGCTAA200.00216183370.4805154
CGCTAAA200.00216183370.4805155
GCAAACG700.067.167391
AAACGCA700.067.1243063
CCGCAAA352.9336265E-453.6994444
CCCGCAA352.9336265E-453.6994443
TGCCCGC1050.049.256081
GTACATG12400.040.5712661
TACATGG12850.039.1504822
ACATGGG12750.038.6951833
GCCCGCA500.00169447337.6137352
CATGGGA4050.037.1255384
GTGTAAA651.4048687E-436.1670531
GTATAAG651.4048687E-436.1670531
GTCCTAC550.002704990834.1943051
ATAAGAC550.002713519134.172373
CATGGGT3650.033.47024
CATGGGG4600.032.6866154
Produced by FastQC (version 0.11.2)