FastQCFastQC Report
Sun 14 Apr 2019
SRR6576352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169355
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4850.28638067963744795No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2460.14525700451713855No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2440.14407605326090164No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2360.13935224823595407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.13462844321100648No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2080.12281893064863748No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2060.12163797939240058No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1860.10982846683003158No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1850.10923799120191313No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1790.10569513743320244No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG1700.1003808567801364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA156.8921404E-493.9994055
CCGCTAA200.002159841170.499564
CCCGCTA250.005228486456.3996433
CATGGGT3950.042.8351754
TGCCCGC802.4364817E-741.136891
ACATGGG11500.040.4606173
TACATGG11700.040.182552
GTACATG11800.039.842021
ATGGGTA1900.039.5786975
GGTACCT1600.038.187268
CATGGGG5300.036.358264
ATAGTAG702.1772558E-433.5712173
GGGTACC1850.033.026827
GTATCAA5250.032.237891
TGGGTAC1900.032.1576926
GGGACGA600.0041511931.3331366
TGTAGGG650.00613089628.9314382
TCTACAC650.00613975228.9228973
GGTTGCA1354.5957495E-727.8516777
AACGCAG6000.027.4164966
Produced by FastQC (version 0.11.2)