FastQCFastQC Report
Sun 14 Apr 2019
SRR6576350_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576350_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213214
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3490.16368531147110413No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2610.12241222433798907No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2600.12194321198420366No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA2160.10130666841764611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTATA250.005233619456.3907132
TATACCG405.670863E-446.992264
GTATCAA3450.043.6997571
AAGGGTA450.001012916641.7708975
GTACATG8750.038.767931
GGTATAG853.9405677E-738.6995057
CTTAGGT1152.757588E-936.7765543
TACATGG9250.036.5777632
TTAGGTA906.166756E-736.5495384
TCAACGC4150.036.2349974
ACATGGG9600.035.2441943
AACGCAG4300.034.9709856
ATCAACG4350.034.569023
GGTAATC550.00271426134.176198
ATACCGT550.00271426134.176196
CAACGCA4400.033.1081855
GTCTTAG1001.3777335E-632.979661
AGGTATA1001.4058387E-632.8945856
GTATAGT1151.1315751E-732.690278
TTAATAC1052.0575953E-631.3281763
Produced by FastQC (version 0.11.2)