FastQCFastQC Report
Sun 14 Apr 2019
SRR6576340_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576340_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345250
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10360.3000724112961622No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6640.19232440260680667No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6340.1836350470673425No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5640.16335988414192612No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5020.14540188269370022No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4870.14105720492396814No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4860.14076755973931931No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4850.1404779145546705No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4650.13468501086169443No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4500.13034033309196233No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4020.1164373642288197No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3990.11556842867487327No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3970.11498913830557567No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3920.11354091238233163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.11209268645908763No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3700.10716871832005792No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3700.10716871832005792No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3660.10601013758146272No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3610.10456191165821868No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3520.10195510499637944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG200.002156835570.549
TAAGGTG500.001701691337.5940934
GTACATG21100.036.1634371
TATTATG651.4115447E-436.15342
GTATCAA9800.036.047261
GTGTAAG801.13929E-535.3263171
TACATGG21850.034.6311532
ACATGGG21500.034.096973
CATGGGT5550.029.6349814
TCACGCA804.778431E-429.3703864
CATGGGG9200.029.114994
AACGCAG12050.028.4685556
TATAAGG1005.3133444E-528.1996542
TGGGTTA856.8126194E-427.6427146
GTGTTAG1554.956746E-827.3494071
ATCAACG12600.027.2258783
CAACGCA12700.027.0115035
ATGGGGA4350.027.007255
TCCGTAC350.00884039526.85292236-37
TCAACGC12850.026.6961944
Produced by FastQC (version 0.11.2)