FastQCFastQC Report
Sun 14 Apr 2019
SRR6576340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345250
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13090.37914554670528605No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7370.21346850108616944No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6380.18479362780593772No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6160.178421433743664No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5580.1616220130340333No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5140.14887762490948586No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4860.14076755973931931No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4750.13758146270818247No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4530.13120926864590876No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4440.12860246198406952No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4430.1283128167994207No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4360.12628530050687906No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4270.12367849384503982No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3970.11498913830557567No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3770.10919623461259956No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3690.10687907313540912No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG3660.10601013758146272No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG3610.10456191165821868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.10079652425778421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10250.042.662641
GTATAAC450.001010780941.7961351
CAACGCA12150.035.9650575
AACGCAG12150.035.9650576
ATCAACG12050.035.873593
TCAACGC12050.035.873594
GTACATG19300.034.1082461
ACATGGG19350.033.2670023
TACATGG20150.032.436082
CATGGGT5400.032.1945274
GTATTAC753.2624145E-431.3471011
ATGGGTA3900.031.3244045
CTAACCG300.00416564931.32440242-43
ACGCAGA14200.030.7729177
CGCAGAG14800.029.5253668
CCCTTAT650.006135101428.9357851
GTATATA650.006135101428.9357851
CATGGGG9900.028.4767284
TATCAAC15700.027.8529992
GTTATAG856.788899E-427.6592061
Produced by FastQC (version 0.11.2)