FastQCFastQC Report
Sun 14 Apr 2019
SRR6576339_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576339_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390213
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11330.29035424242657215No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7450.1909213685858749No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6670.1709322857003739No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6130.15709368985656552No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5490.14069239107871856No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5300.13582325550404523No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5080.13018530904916034No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4840.12403482200746771No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4460.11429655085812107No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4440.11378401027131335No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4430.1135277399779095No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4210.1078897935230246No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4210.1078897935230246No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4200.10763352322962075No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4140.10609590146919759No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4110.10532709058898601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8950.045.789151
ATCAACG11950.034.6125763
TCAACGC12100.034.5719454
CAACGCA12850.032.9199035
AACGCAG12950.032.6656956
GTACATG21550.032.5690731
TACATGG22550.031.4738272
ACATGGG21850.031.4065763
CATGGGT6350.029.6077524
ATGGGTA4650.028.3024655
CGCAGAG15400.027.465368
ACGCAGA15450.027.0722927
GGTACCT4250.026.5390788
GTATCAT909.383398E-426.1694071
TATCAAC16000.025.8512692
CATGGGG11350.025.6752684
GCAGAGT17000.024.8803849
TGAATAG950.001302669224.7380585
ATATGAC1000.001756105123.5011543
GCCTTAG1252.3831955E-422.6103671
Produced by FastQC (version 0.11.2)