FastQCFastQC Report
Sun 14 Apr 2019
SRR6576338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328557
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12590.3831907401151094No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7050.21457464001680074No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6090.18535596563153425No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5580.16983354486436142No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5570.16952918367284822No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5210.15857218077837332No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4830.147006455500872No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4670.14213667643666092No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4320.13148403473369916No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4300.1308753123506728No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4290.13057095115915962No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4270.12996222877613323No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4250.12935350639310683No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4090.12448372732889573No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4030.12265756017981659No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3710.11291800205139443No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3710.11291800205139443No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG3610.1098743901362625No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC3480.105917694646591No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3470.1056133334550778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.10317844392297226No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3340.10165663796540632No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3310.10074355439086674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG651.4123158E-436.1493951
GGTACCT3750.035.0890168
ATGGGTA4100.034.3860135
GTACTAT702.1831978E-433.5672951
GTACATG22350.033.2218631
ACATGGG22100.032.534463
TACATGG22800.032.1588332
CATGGGT6050.031.8473194
CCTTATA600.004156103431.3342442
GTATCAA11300.031.1908511
CATGGGG8100.030.1691284
CTATAGT650.006151557428.9195161
GTTCTAG1354.622707E-727.8484251
TGGGTAC4800.027.413296
GGGTACC4800.027.413297
TGTAATA700.00882574926.8579252
GTCTAAA700.00883230526.8538361
TATACTG700.00883230526.8538365
GCCCATA909.509993E-426.1078991
ATGGGAG3800.025.9704885
Produced by FastQC (version 0.11.2)