FastQCFastQC Report
Sun 14 Apr 2019
SRR6576337_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576337_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374669
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11540.3080051992558765No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6900.18416255414779445No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6160.16441178747107446No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5820.1553371108898788No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5390.14386031403719018No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5370.14332650953241394No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5010.13371802844644207No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4910.13104900592256097No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4810.1283799833986799No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4620.12330884060330585No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4610.12304193835091776No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4330.11556867528405071No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4260.11370035951733395No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3940.10515948744091452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.10329117167419777No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3830.10222356266464533No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3800.101422855907481No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3780.10088905140270478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10450.041.8934171
ACATGGG23650.035.7752233
GTACATG23950.035.772151
AACGCAG12700.035.1609766
TACATGG24650.034.5145872
CCCCTAT550.002693877334.235481
CAACGCA13200.034.185225
ATCAACG13600.033.5253873
TCAACGC13550.032.9553074
CATGGGG10600.031.9277024
ACGCAGA14600.030.5811487
CGCAGAG14500.030.4679288
ATGGGAG4350.030.2558825
ATGGGCG804.7720256E-429.377925
CATGGGA9850.028.155084
CATGGGT6200.028.0511744
GTATTAC856.745295E-427.6904621
TCTATAC856.8035E-427.6498053
CTATAGT700.0088242826.8598124
CTCCCGT700.0088242826.8598126
Produced by FastQC (version 0.11.2)