FastQCFastQC Report
Sun 14 Apr 2019
SRR6576336_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576336_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences676244
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17830.26366222842642595No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11250.16636007121689803No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11030.1631068076019898No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9580.14166484286736739No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9470.1400382110599133No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9250.13678494744500505No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8970.13264443011694005No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG8610.1273209078379993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8400.12421551984195055No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG8260.12214526117791803No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7920.11711749013669623No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC7890.11667386328011783No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC7760.11475148023494477No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7680.11356847528406908No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7570.11194184347661496No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT7230.10691407243539314No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7130.10543531624679849No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT6840.10114692329987401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13300.044.3351171
GGTATCA6250.040.75711
TACATGG34050.036.861352
GTACATG34500.036.64421
ACATGGG35550.035.0311973
GGGTACC6600.032.7538647
TGGGTAC6800.031.7905146
ATCAACG19000.031.164893
ATGGGTA7550.031.1223035
TCAACGC19200.030.8402544
TTAGGTA2450.030.69044
CAACGCA19250.030.5160245
GGTACCT7100.029.7853588
AACGCAG19800.029.6683546
TAGGTAT2950.028.6747195
CATGGGT10700.028.1089674
AGGTATA3100.027.2872316
CATGGGG21400.027.010964
GACTTAC700.00867699926.9557551
ATAGTAT1606.914706E-826.4345046
Produced by FastQC (version 0.11.2)