FastQCFastQC Report
Sun 14 Apr 2019
SRR6576333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258179
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4710.18243156879529318No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3280.1270436402650874No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2980.1154237951188904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.10767723168809237No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2690.10419127814423326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10050.039.7478871
GTACATG19500.037.355821
TACATGG19750.036.1690942
ACATGGG20250.035.26923
TATACAG1204.1909516E-935.2401925
CATGGGT4450.034.844244
AACGCAG11350.034.7744676
ATCAACG11350.034.3604853
GCATAGA550.00271424934.178931
TCGCAAA550.002716828834.1723194
CAACGCA11800.033.4483225
TCAACGC11950.033.028474
TATCAAC12700.031.4540332
GACTAAA600.00415677931.3306871
GATTTAT1052.0585303E-631.3306851
ACGCAGA12800.030.8351697
CGCAGAG13200.029.9007728
CAATACT804.7785146E-429.3668294
GTCTAAC650.00614798728.9206331
CATGGGA10500.028.1921564
Produced by FastQC (version 0.11.2)