FastQCFastQC Report
Sun 14 Apr 2019
SRR6576331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244747
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4680.19121786988195974No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2810.11481243896758694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG451.605176E-552.2497141
GTACATG15700.036.541521
TACATGG15850.035.602332
GTATCAA8350.034.9165761
CCGTCCA550.00271672834.1718649
ACATGGG16400.034.0937233
GGGTACC1800.033.9345557
CATGGGG5600.033.561654
AACGCAG9050.031.670336
ATGGGAG3000.029.7579985
ATCAACG9750.029.3965633
GCTAGAC804.778162E-429.3664423
CAACGCA9800.029.2465825
GGTACCT2101.8189894E-1229.0867638
TCAACGC9900.028.951164
AAACGCA650.00615358728.9146543
CATGGGT3950.028.5486434
ACGCAGA10250.027.9625847
ATGGGTA2203.6379788E-1227.7646375
TGGGTAC2253.6379788E-1227.1476466
Produced by FastQC (version 0.11.2)